解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 330-333.doi: 10.3969/j.issn.0529-1356.2013.03.007

• 细胞和分子生物学 • 上一篇    下一篇

表没食子儿茶素-3-没食子酸酯抑制烫伤诱导的小鼠皮肤巨噬细胞炎症蛋白-2的表达

李嘉雯 崔海燕 张东芳 肖鹏 李卫国*   

  1. 河南师范大学生命科学学院, 河南 新乡 453007
  • 收稿日期:2012-08-10 修回日期:2012-09-03 出版日期:2013-06-06 发布日期:2013-07-16
  • 通讯作者: 李卫国 E-mail:liwg0618@htu.cn
  • 基金资助:

    河南省重点科技攻关计划（112102300320）；河南省教育厅自然科学基础研究计划（2010A180011）;其他(不属于以上基金类别的请自行输入下框)

Inhibition of epigallocatechin-3-gallate on scald-induced macrophage inflammatory protein-2 expression in the skin of mice

LI Jia-wen  CUI Hai-yan  ZHANG Dong-fang  XIAO Peng  LI Wei-guo*   

  1. College of Life Science, He’nan Normal University, He’nan Xinxiang 453007, China
  • Received:2012-08-10 Revised:2012-09-03 Online:2013-06-06 Published:2013-07-16

摘要：

目的 探讨表没食子儿茶素-3-没食子酸酯(EGCG)对局部烫伤诱导的小鼠皮肤组织巨噬细胞炎症蛋白-2（MIP-2）表达的影响 方法将
52只昆明种小鼠随机分为空白对照组、烫伤组和烫伤EGCG处理组，其中48只小鼠进行局部皮肤烫伤。利用实时定量PCR和ELISA法检测皮肤组织
MIP-2 mRNA和MIP-2蛋白水平。结果 局部烫伤可引起受损部位皮肤组织的MIP-2 mRNA和MIP-2水平升高，而涂敷0.2g/kg EGCG膏剂可使MIP-2表
达水平下降。结论 0.2g/kg EGCG膏剂抑制局部烫伤诱导的皮肤组织MIP-2表达，减弱由MIP-2引起的炎症反应，促进受损皮肤修复。

关键词: 烫伤, 皮肤, 表没食子儿茶素-3-没食子酸酯, 巨噬细胞炎症蛋白-2, 实时定量聚合酶链反应, 酶联免疫吸附法, 小鼠

Abstract:

Objective To investigate influence of epigallocatechin-3-gallate (EGCG) on expression of macrophage inflammatory
protein-2(MIP-2) induced by locally scalded skin in mice. Methods Fity-two Kunming mice were divided into the control, scalded
skin, and EGCG groups. Forty-eight mice were used to establish a skin scalded model. The real time quantitative PCR (qRT-PCR)
and ELISA were used to evaluate MIP-2 expression in cutaneous tissue at both the mRNA and protein expression levels. Results
Both MIP-2 mRNA and protein levels in cutaneous tissue at injury sites increased after skin was scalded, but both decreased after
the scalded skin was treated by 0.2g/kg EGCG. Conclusion The data suggest that 0.2g/kg EGCG may suppress MIP-2 expression stimulated
by local skin scald, and decrease inflammatory reaction induced by MIP-2 release in cutaneous tissue at injury sites, thus EGCG
is able to promote the repair of skin injury elicited by scald.

Key words: Scald, Skin, Epigallocatechin-3-gallate, Macrophage inflammatory protein-2, qRT-PCR, ELISA, Mouse

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